Oligoclonal expansion and CD1 recognition by human intestinal intraepithelial lymphocytes
SP Balk, EC Ebert, RL Blumenthal, FV McDermott… - Science, 1991 - science.org
SP Balk, EC Ebert, RL Blumenthal, FV McDermott, KW Wucherpfennig, SB Landau…
Science, 1991•science.orgA human intestinal intraepithelial lymphocyte (IEL) T cell line was established from jejunum
to characterize the structure and function of the αβ T cell antigen receptors (TCRs)
expressed by this population. Single-sided polymerase chain reaction (PCR) amplification
cloning and quantitative PCR amplification of the TCR chains from the cell line and from
fresh IELs demonstrated that IELs were oligoclonal. The IEL T cell line exhibited CD1-
specific cytotoxicity and a dominant IEL T cell clone was CD1c-specific. Thus, human jejunal …
to characterize the structure and function of the αβ T cell antigen receptors (TCRs)
expressed by this population. Single-sided polymerase chain reaction (PCR) amplification
cloning and quantitative PCR amplification of the TCR chains from the cell line and from
fresh IELs demonstrated that IELs were oligoclonal. The IEL T cell line exhibited CD1-
specific cytotoxicity and a dominant IEL T cell clone was CD1c-specific. Thus, human jejunal …
A human intestinal intraepithelial lymphocyte (IEL) T cell line was established from jejunum to characterize the structure and function of the αβ T cell antigen receptors (TCRs) expressed by this population. Single-sided polymerase chain reaction (PCR) amplification cloning and quantitative PCR amplification of the TCR chains from the cell line and from fresh IELs demonstrated that IELs were oligoclonal. The IEL T cell line exhibited CD1-specific cytotoxicity and a dominant IEL T cell clone was CD1c-specific. Thus, human jejunal intraepithelial lymphocytes are oligoclonal and recognize members of the CD1 gene family.
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