Formation of leukotrienes and other hydroxy acids during platelet-neutrophil interactions in vitro

AJ Marcus, MJ Broekman, LB Safier, HL Ullman… - Biochemical and …, 1982 - Elsevier
AJ Marcus, MJ Broekman, LB Safier, HL Ullman, N Islam, CN Serhan, LE Rutherford…
Biochemical and biophysical research communications, 1982Elsevier
Interactions of human platelets with neutrophils were studied in suspensions of [3 H]
arachidonate-labeled platelets and unlabeled neutrophils stimulated with ionophore
A23187. Several radioactive arachidonate metabolites, not produced by platelets alone,
were detected, including [3 H]-labeled leukotriene B 4 (LTB 4), dihydroxyeicosatetraenoic
acid (DHETE) and 5-hydroxyeicosatetraenoic acid (5-HETE). When [3 H] 12-HETE, a
platelet product, was added to stimulated neutrophils, DHETE was formed. Similarly, when …
Abstract
Interactions of human platelets with neutrophils were studied in suspensions of [3H]arachidonate-labeled platelets and unlabeled neutrophils stimulated with ionophore A23187. Several radioactive arachidonate metabolites, not produced by platelets alone, were detected, including [3H]-labeled leukotriene B4 (LTB4), dihydroxyeicosatetraenoic acid (DHETE) and 5-hydroxyeicosatetraenoic acid (5-HETE). When [3H]12-HETE, a platelet product, was added to stimulated neutrophils, DHETE was formed. Similarly, when [3H]5-HETE, a neutrophil product, was added to stimulated platelets, DHETE was the major product. These results suggest that upon stimulation: 1) platelet-derived arachidonate may serve as precursor for the neutrophil-derived eicosanoids LTB4 and 5-HETE, and 2) that platelet-derived 12-HETE can be converted to DHETE by human neutrophils. The present investigation documents cell-cell interactions via the lipoxygenase pathway, which may be important in hemostasis, thrombosis and inflammation.
Elsevier