[HTML][HTML] Reconstitution of sterol-regulated endoplasmic reticulum-to-Golgi transport of SREBP-2 in insect cells by co-expression of mammalian SCAP and Insigs
IY Dobrosotskaya, JL Goldstein, MS Brown… - Journal of Biological …, 2003 - ASBMB
In mammalian cells, membrane-bound sterol regulatory element-binding proteins (SREBPs)
are transported from ER to Golgi where they are processed proteolytically to generate
soluble transcription factors that activate lipid synthesis. ER-to-Golgi transport requires
SCAP, a sterol-regulated escort protein. In sterol-treated cells, the SCAP/SREBP complex
binds to Insig-1 or Insig-2, which retains the complex in the ER, blocking SREBP processing
and decreasing lipid synthesis. In Drosophila cells, the endogenous SCAP/SREBP complex …
are transported from ER to Golgi where they are processed proteolytically to generate
soluble transcription factors that activate lipid synthesis. ER-to-Golgi transport requires
SCAP, a sterol-regulated escort protein. In sterol-treated cells, the SCAP/SREBP complex
binds to Insig-1 or Insig-2, which retains the complex in the ER, blocking SREBP processing
and decreasing lipid synthesis. In Drosophila cells, the endogenous SCAP/SREBP complex …