[HTML][HTML] Consequences of mevalonate depletion: differential transcriptional, translational, and post-translational up-regulation of Ras, Rap1a, RhoA, and RhoB

SA Holstein, CL Wohlford-Lenane, RJ Hohl - Journal of Biological …, 2002 - ASBMB
Ras-related proteins are small GTPases that are post-translationally modified with
mevalonate-derived isoprenoids. Although the effects of inhibition of isoprenylation on
protein function have been examined, the consequences of depletion of isoprenoid pools on
regulation of expression of isoprenylated proteins have yet to be investigated. In these
studies we have shown that depletion of mevalonate results in increased total levels of Ras,
Rap1a, RhoA, and RhoB in K562 cells. Cycloheximide and [35 S] methionine pulse/pulse …