A rapid optimized protocol for downward alkaline Southern blotting of DNA.
PA Koetsier, J Schorr, W Doerfler - BioTechniques, 1993 - europepmc.org
PA Koetsier, J Schorr, W Doerfler
BioTechniques, 1993•europepmc.orgA rapid optimized protocol for downward alkaline Southern blotting of DNA. - Abstract - Europe
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A simple and efficient 2.5-h Southern blotting procedure is described that uses 0.4 M NaOH to transfer DNA in a downward direction. The resulting blots give signals that are both sharper and 30% stronger than those obtained by conventional upward-transferred blots.
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