[HTML][HTML] Cloning of a third mammalian Na+-Ca2+ exchanger, NCX3
DA Nicoll, BD Quednau, Z Qui, YR Xia, AJ Lusis… - Journal of Biological …, 1996 - ASBMB
NCX3 is the third isoform of a mammalian Na+-Ca 2+ exchanger to be cloned. NCX3 was
identified from rat brain cDNA by polymerase chain reaction (PCR) using degenerate
primers derived from the sequences of two conserved regions of NCX1 and NCX2. The
NCX3 PCR product was used to isolate two overlapping clones totalling 4.8 kilobases (kb)
from a rat brain cDNA library. The overlapping clones were sequenced and joined at a
unique Bsp106I restriction enzyme site to form a full-length cDNA clone. The NCX3 cDNA …
identified from rat brain cDNA by polymerase chain reaction (PCR) using degenerate
primers derived from the sequences of two conserved regions of NCX1 and NCX2. The
NCX3 PCR product was used to isolate two overlapping clones totalling 4.8 kilobases (kb)
from a rat brain cDNA library. The overlapping clones were sequenced and joined at a
unique Bsp106I restriction enzyme site to form a full-length cDNA clone. The NCX3 cDNA …