We constructed an animal model of stimulated single‐fiber electromyography (SFEMG) by testing Wistar rats under anesthesia. Stimuli of 1 Hz were applied to the sciatic nerve through an insulated monopolar needle electrode. Single‐fiber action potentials were acquired from the gastrocnemius muscle. Jitter was assessed by the mean consecutive difference (MCD). Eighty‐seven fibers were obtained from 12 rats. Their MCDs ranged from 2 to 72 μs (17.7 ± 13.4). Seven of these values were less than or equal to 5 μs, and three exceeded 50 μs. Neuromuscular blocking agents injected into some of the rats induced considerable increases in jitter and blocking. A rat with one fiber with an MCD less than 5 μs also received an injection of curare. The jitter showed the same pattern of increment, evidence that the small jitter was not attributable to direct muscle stimulation. These results show that SFEMG can be used on rats. In addition, jitter reflects the changes in motor end‐plate function. The findings also suggest the presence of an extremely high safety factor in rat neuromuscular junctions. © 1998 John Wiley & Sons, Inc. Muscle Nerve 21:482–489, 1998.