Effect of insulin on amino acid uptake and protein turnover in skeletal muscle from septic rats: evidence for insulin resistance of protein breakdown

PO Hasselgren, BW Warner, JH James… - Archives of …, 1987 - jamanetwork.com
• We investigated the effect of different concentrations of insulin (0, 10, 1× 10 2, 1× 10 3, 1×
10 4, and 1× 10 5 mU/L [0, 70, 7× 10 2, 7× 10 3, 7× 10 4, and 7× 10 5 pmol/L]) on amino acid
(α-aminoisobutyric acid) uptake and protein synthesis and breakdown in incubated extensor
digitorum longus (EDL) and soleus muscles of rats. We studied three groups:(1) untreated,
fed rats;(2) sham-operated rats; and (3) septic rats. Sepsis was induced by cecal ligation and
puncture. The α-aminoisobutyric acid uptake was increased by insulin in all three groups …