Isolation of autophagocytosis mutants of Saccharomyces cerevisiae
M Thumm, R Egner, B Koch, M Schlumpberger… - FEBS letters, 1994 - Elsevier
Protein degradation in the vacuole (lysosome) is an important event in cellular regulation. In
yeast, as in mammalian cells, a major route of protein uptake for degradation into the
vacuole (lysosome) has been found to be autophagocytosis. The discovery of this process in
yeast enables the elucidation of its mechanisms via genetic and molecular biological
investigations. Here we report the isolation of yeast mutants defective in autophagocytosis
(aut mutants), using a rapid colony screening procedure.
yeast, as in mammalian cells, a major route of protein uptake for degradation into the
vacuole (lysosome) has been found to be autophagocytosis. The discovery of this process in
yeast enables the elucidation of its mechanisms via genetic and molecular biological
investigations. Here we report the isolation of yeast mutants defective in autophagocytosis
(aut mutants), using a rapid colony screening procedure.
Abstract
Protein degradation in the vacuole (lysosome) is an important event in cellular regulation. In yeast, as in mammalian cells, a major route of protein uptake for degradation into the vacuole (lysosome) has been found to be autophagocytosis. The discovery of this process in yeast enables the elucidation of its mechanisms via genetic and molecular biological investigations. Here we report the isolation of yeast mutants defective in autophagocytosis (aut mutants), using a rapid colony screening procedure.
Elsevier