Isolation and Characterization of a sigBDeletion Mutant of Staphylococcus aureus
RO Nicholas, T Li, D McDevitt, A Marra… - Infection and …, 1999 - Am Soc Microbiol
RO Nicholas, T Li, D McDevitt, A Marra, S Sucoloski, PL Demarsh, DR Gentry
Infection and immunity, 1999•Am Soc MicrobiolThe sigB gene of Staphylococcus aureus, coding for the alternate sigma factor B, has been
deleted by allelic replacement mutagenesis. The mutant grew as well as the parent in vitro,
although it was deficient in clumping factor, coagulase, and pigment. In two murine and one
rat infection model the mutant showed no reduction in virulence.
deleted by allelic replacement mutagenesis. The mutant grew as well as the parent in vitro,
although it was deficient in clumping factor, coagulase, and pigment. In two murine and one
rat infection model the mutant showed no reduction in virulence.
Abstract
The sigB gene of Staphylococcus aureus, coding for the alternate sigma factor B, has been deleted by allelic replacement mutagenesis. The mutant grew as well as the parent in vitro, although it was deficient in clumping factor, coagulase, and pigment. In two murine and one rat infection model the mutant showed no reduction in virulence.
American Society for Microbiology