[HTML][HTML] Structure-function studies of interleukin 15 using site-specific mutagenesis, polyethylene glycol conjugation, and homology modeling
DK Pettit, TP Bonnert, J Eisenman, S Srinivasan… - Journal of Biological …, 1997 - ASBMB
Interleukin (IL)-15 is a multifunctional cytokine that shares many biological activities with IL-
2. This functional overlap, as well as receptor binding subunits shared by IL-15 and IL-2,
suggests tertiary structural similarities between these two cytokines. In this study,
recombinant human IL-15 was PEGylated via lysine-specific conjugation chemistry in order
to extend the circulation half-life of this cytokine. Although PEGylation did extend the β-
elimination circulation half-life of IL-15 by greater than 50-fold, the biological activity of …
2. This functional overlap, as well as receptor binding subunits shared by IL-15 and IL-2,
suggests tertiary structural similarities between these two cytokines. In this study,
recombinant human IL-15 was PEGylated via lysine-specific conjugation chemistry in order
to extend the circulation half-life of this cytokine. Although PEGylation did extend the β-
elimination circulation half-life of IL-15 by greater than 50-fold, the biological activity of …