Isolation of Rat Pancreatic Islets by Ductal Injection of collagenase1

R Sutton, M PETERS, P MCSHANE, DWR GRAY… - …, 1986 - journals.lww.com
R Sutton, M PETERS, P MCSHANE, DWR GRAY, PJ MORRIS
Transplantation, 1986journals.lww.com
This study demonstrates that biliary obstruction results in impairment of lymphoproliferative
response to T cell dependent, but not T cell independent mitogen in vitro. This suggests an
impairment of cellular but not humoral immune mechanisms in this model. Suppression of
cellular immune mechanisms, demonstrated as an impaired host-versus-graft response to
allogeneic F, lymphoid cells was apparent in BDL animals in vivo. This demonstrates an
impaired effector response to histoincompatible tissue in the cholestatic host. In vitro …
This study demonstrates that biliary obstruction results in impairment of lymphoproliferative response to T cell dependent, but not T cell independent mitogen in vitro. This suggests an impairment of cellular but not humoral immune mechanisms in this model. Suppression of cellular immune mechanisms, demonstrated as an impaired host-versus-graft response to allogeneic F, lymphoid cells was apparent in BDL animals in vivo. This demonstrates an impaired effector response to histoincompatible tissue in the cholestatic host. In vitro mitogen studies failed to demonstrate lymphocytic suppressive activity in sera related from cholestatic animals or elevated levels of immunoregulatory a globulin fractions in this model. Eluci-
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