[HTML][HTML] Identification of an Elastin Cross-linking Domain That Joins Three Peptide Chains: POSSIBLE ROLE IN NUCLEATED ASSEMBLY (∗)
P Brown-Augsburger, C Tisdale, T Broekelmann… - Journal of Biological …, 1995 - ASBMB
The alignment of elastin molecules in the mature elastic fiber was investigated by purifying
and sequencing cross-link-containing peptides generated by proteolytic digestion of
incompletely cross-linked insoluble elastin. Peptides of interest were purified by reverse
phase and size exclusion high performance liquid chromatography and characterized by
amino acid analysis and protein sequencing. One peptide, consisting of the cross-linking
domain encoded by exon 10, contained a modified lysine residue that had not condensed to …
and sequencing cross-link-containing peptides generated by proteolytic digestion of
incompletely cross-linked insoluble elastin. Peptides of interest were purified by reverse
phase and size exclusion high performance liquid chromatography and characterized by
amino acid analysis and protein sequencing. One peptide, consisting of the cross-linking
domain encoded by exon 10, contained a modified lysine residue that had not condensed to …