Diphenyleneiodonium sulfate, an NADPH oxidase inhibitor, prevents early alcohol-induced liver injury in the rat

H Kono, I Rusyn, T Uesugi… - American Journal …, 2001 - journals.physiology.org
H Kono, I Rusyn, T Uesugi, S Yamashina, HD Connor, A Dikalova, RP Mason, RG Thurman
American Journal of Physiology-Gastrointestinal and Liver …, 2001journals.physiology.org
The oxidant source in alcohol-induced liver disease remains unclear. NADPH oxidase
(mainly in liver Kupffer cells and infiltrating neutrophils) could be a potential free radical
source. We aimed to determine if NADPH oxidase inhibitor diphenyleneiodonium sulfate
(DPI) affects nuclear factor-κB (NF-κB) activation, liver tumor necrosis factor-α (TNF-α)
mRNA expression, and early alcohol-induced liver injury in rats. Male Wistar rats were fed
high-fat liquid diets with or without ethanol (10–16 g· kg− 1· day− 1) continuously for up to 4 …
The oxidant source in alcohol-induced liver disease remains unclear. NADPH oxidase (mainly in liver Kupffer cells and infiltrating neutrophils) could be a potential free radical source. We aimed to determine if NADPH oxidase inhibitor diphenyleneiodonium sulfate (DPI) affects nuclear factor-κB (NF-κB) activation, liver tumor necrosis factor-α (TNF-α) mRNA expression, and early alcohol-induced liver injury in rats. Male Wistar rats were fed high-fat liquid diets with or without ethanol (10–16 g · kg−1 · day−1) continuously for up to 4 wk, using the Tsukamoto-French intragastric enteral feeding protocol. DPI or saline vehicle was administered by subcutaneous injection for 4 wk. Mean urine ethanol concentrations were similar between the ethanol- and ethanol plus DPI-treated groups. Enteral ethanol feeding caused severe fat accumulation, mild inflammation, and necrosis in the liver (pathology score, 4.3 ± 0.3). In contrast, DPI significantly blunted these changes (pathology score, 0.8 ± 0.4). Enteral ethanol administration for 4 wk also significantly increased free radical adduct formation, NF-κB activity, and TNF-α expression in the liver. DPI almost completely blunted these parameters. These results indicate that DPI prevents early alcohol-induced liver injury, most likely by inhibiting free radical formation via NADPH oxidase, thereby preventing NF-κB activation and TNF-α mRNA expression in the liver.
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