The separation of the granulocytes from different rat strains A comparative study
S Szücs, C Varga, I Ember, P Kertai - Journal of immunological methods, 1994 - Elsevier
Different Percoll density gradients were used to purify granulocytes from Long-Evans,
Fischer, Sprague-Dawley, and Fischer/Long Evans hybrid rats. Three different discontinuous
gradient types were developed which permitted the separation of polymorphonuclear
leukocytes (PMNLs) from the peripheral blood of the different rat strains. Our purification
techniques were compared to each other in terms of purity and yield. Purities of 97.7±0.6%,
97.0±1.1%, 96.4±1.2% and 96.7%±1.1% were achieved for the granulocyte fractions of LE …
Fischer, Sprague-Dawley, and Fischer/Long Evans hybrid rats. Three different discontinuous
gradient types were developed which permitted the separation of polymorphonuclear
leukocytes (PMNLs) from the peripheral blood of the different rat strains. Our purification
techniques were compared to each other in terms of purity and yield. Purities of 97.7±0.6%,
97.0±1.1%, 96.4±1.2% and 96.7%±1.1% were achieved for the granulocyte fractions of LE …