Protein kinase C isotypes controlled by phosphoinositide 3-kinase through the protein kinase PDK1
JA Le Good, WH Ziegler, DB Parekh, DR Alessi… - Science, 1998 - science.org
Phosphorylation sites in members of the protein kinase A (PKA), PKG, and PKC kinase
subfamily are conserved. Thus, the PKB kinase PDK1 may be responsible for the
phosphorylation of PKC isotypes. PDK1 phosphorylated the activation loop sites of PKCζ
and PKCδ in vitro and in a phosphoinositide 3-kinase (PI 3-kinase)–dependent manner in
vivo in human embryonic kidney (293) cells. All members of the PKC family tested formed
complexes with PDK1. PDK1-dependent phosphorylation of PKCδ in vitro was stimulated by …
subfamily are conserved. Thus, the PKB kinase PDK1 may be responsible for the
phosphorylation of PKC isotypes. PDK1 phosphorylated the activation loop sites of PKCζ
and PKCδ in vitro and in a phosphoinositide 3-kinase (PI 3-kinase)–dependent manner in
vivo in human embryonic kidney (293) cells. All members of the PKC family tested formed
complexes with PDK1. PDK1-dependent phosphorylation of PKCδ in vitro was stimulated by …
Phosphorylation sites in members of the protein kinase A (PKA), PKG, and PKC kinase subfamily are conserved. Thus, the PKB kinase PDK1 may be responsible for the phosphorylation of PKC isotypes. PDK1 phosphorylated the activation loop sites of PKCζ and PKCδ in vitro and in a phosphoinositide 3-kinase (PI 3-kinase)–dependent manner in vivo in human embryonic kidney (293) cells. All members of the PKC family tested formed complexes with PDK1. PDK1-dependent phosphorylation of PKCδ in vitro was stimulated by combined PKC and PDK1 activators. The activation loop phosphorylation of PKCδ in response to serum stimulation of cells was PI 3-kinase–dependent and was enhanced by PDK1 coexpression.
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