Active and latent forms of transforming growth factor beta activity in synovial effusions.
R Fava, N Olsen, J Keski-Oja, H Moses… - The Journal of …, 1989 - rupress.org
The Journal of experimental medicine, 1989•rupress.org
We have evaluated the possible involvement of TGF-beta in rheumatoid arthritis by assay of
16 cell-free synovial fluids for the presence of its active and" latent" forms. Evidence has
been obtained for TGF-beta-like activity in synovial effusions by four criteria:(a) TGF-beta
receptor competition,(b) soft-agar colony formation of AKR-2B and NRK-49F indicator
cells,(c) immunological neutralization of the biological activity, and (d) biochemical activation
of a latent form.
16 cell-free synovial fluids for the presence of its active and" latent" forms. Evidence has
been obtained for TGF-beta-like activity in synovial effusions by four criteria:(a) TGF-beta
receptor competition,(b) soft-agar colony formation of AKR-2B and NRK-49F indicator
cells,(c) immunological neutralization of the biological activity, and (d) biochemical activation
of a latent form.
We have evaluated the possible involvement of TGF-beta in rheumatoid arthritis by assay of 16 cell-free synovial fluids for the presence of its active and "latent" forms. Evidence has been obtained for TGF-beta-like activity in synovial effusions by four criteria: (a) TGF-beta receptor competition, (b) soft-agar colony formation of AKR-2B and NRK-49F indicator cells, (c) immunological neutralization of the biological activity, and (d) biochemical activation of a latent form.
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