Myogenicity in vitro and in vivo of mouse muscle cells separated on discontinuous Percoll gradients
JE Morgan - Journal of the Neurological Sciences, 1988 - Elsevier
Mouse muscle cells, obtained by enzymatically disaggregating newborn mouse muscle,
were separated on a discontinuous Percoll gradient. The myogenicity in vitro of the resultant
cell fractions was examined by counting the percentage of nuclei in myotubes. Myogenicity
in vivo was assessed by implanting a cell suspension of one of the allotypes of glucose-6-
phosphate isomerase (GPI) into a regenerating skeletal muscle graft of a second GPI
allotype: the finding of hybrid GPI indicated that the implanted cells were myogenic …
were separated on a discontinuous Percoll gradient. The myogenicity in vitro of the resultant
cell fractions was examined by counting the percentage of nuclei in myotubes. Myogenicity
in vivo was assessed by implanting a cell suspension of one of the allotypes of glucose-6-
phosphate isomerase (GPI) into a regenerating skeletal muscle graft of a second GPI
allotype: the finding of hybrid GPI indicated that the implanted cells were myogenic …