Dual recognition of double‐stranded DNA by 2′‐aminoethoxy‐modified oligonucleotides
B Cuenoud, F Casset, D Hüsken, F Natt… - Angewandte Chemie …, 1998 - Wiley Online Library
Angewandte Chemie International Edition, 1998•Wiley Online Library
Simultaneous interaction of the 2′‐aminoethoxy‐modified oligonucleotides with the
phosphodiester backbone (shown on the right, A) and with the bases through Hoogsteen
base contacts (B) is seen at each base‐pair step of the duplex DNA target. The electrostatic
interaction between the protonated amino group and the negatively charged phosphate
group provides for a dramatic increase in the binding affinity and the association rate
constant.
phosphodiester backbone (shown on the right, A) and with the bases through Hoogsteen
base contacts (B) is seen at each base‐pair step of the duplex DNA target. The electrostatic
interaction between the protonated amino group and the negatively charged phosphate
group provides for a dramatic increase in the binding affinity and the association rate
constant.
Abstract
Simultaneous interaction of the 2′‐aminoethoxy‐modified oligonucleotides with the phosphodiester backbone (shown on the right, A) and with the bases through Hoogsteen base contacts (B) is seen at each base‐pair step of the duplex DNA target. The electrostatic interaction between the protonated amino group and the negatively charged phosphate group provides for a dramatic increase in the binding affinity and the association rate constant.
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