Replication of the human hepatitis delta virus genome is initiated in mouse hepatocytes following intravenous injection of naked DNA or RNA sequences
As early as 5 days after DNA copies of the hepatitis delta virus (HDV) genome or even in
vitro-transcribed HDV RNA sequences were injected into the mouse tail vein using the
hydrodynamics-based transfection procedure of F. Liu et al.(Gene Ther. 6: 1258–1266,
1999), it was possible to detect in the liver by Northern analyses of RNA, immunoblots of
protein, and immunostaining of liver sections what were considered typical features of HDV
genome replication. This transfection strategy should have valuable applications for in vivo …
vitro-transcribed HDV RNA sequences were injected into the mouse tail vein using the
hydrodynamics-based transfection procedure of F. Liu et al.(Gene Ther. 6: 1258–1266,
1999), it was possible to detect in the liver by Northern analyses of RNA, immunoblots of
protein, and immunostaining of liver sections what were considered typical features of HDV
genome replication. This transfection strategy should have valuable applications for in vivo …
Abstract
As early as 5 days after DNA copies of the hepatitis delta virus (HDV) genome or even in vitro-transcribed HDV RNA sequences were injected into the mouse tail vein using the hydrodynamics-based transfection procedure of F. Liu et al. (Gene Ther. 6:1258–1266, 1999), it was possible to detect in the liver by Northern analyses of RNA, immunoblots of protein, and immunostaining of liver sections what were considered typical features of HDV genome replication. This transfection strategy should have valuable applications for in vivo studies of HDV replication and pathogenesis and may also be useful for studies of other hepatotropic viruses.
American Society for Microbiology