[HTML][HTML] Urokinase plasminogen activator and gelatinases are associated with membrane vesicles shed by human HT1080 fibrosarcoma cells
A Ginestra, S Monea, G Seghezzi, V Dolo… - Journal of Biological …, 1997 - ASBMB
Membrane vesicles are shed by tumor cells bothin vivo and in vitro. Although their functions
are not well understood, it has been proposed that they may play multiple roles in tumor
progression. We characterized membrane vesicles from human HT1080 fibrosarcoma cell
cultures for the presence of proteinases involved in tumor invasion. By gelatin zymography
and Western blotting, these vesicles showed major bands corresponding to the zymogen
and active forms of gelatinase B (MMP-9) and gelatinase A (MMP-2) and to the MMP-9 …
are not well understood, it has been proposed that they may play multiple roles in tumor
progression. We characterized membrane vesicles from human HT1080 fibrosarcoma cell
cultures for the presence of proteinases involved in tumor invasion. By gelatin zymography
and Western blotting, these vesicles showed major bands corresponding to the zymogen
and active forms of gelatinase B (MMP-9) and gelatinase A (MMP-2) and to the MMP-9 …