Characterization of 5′ AMP-activated protein kinase activity in the heart and its role in inhibiting acetyl-CoA carboxylase during reperfusion following ischemia
N Kudo, JG Gillespie, L Kung, LA Witters… - … et Biophysica Acta (BBA …, 1996 - Elsevier
Despite the high expression of 5′ AMP activated protein kinase (AMPK) in heart, the activity
and function of this enzyme in heart muscle has not been characterized. We demonstrate
that rat hearts have a high AMPK activity, comparable to that found in liver, which could be
stimulated up to 3-fold by 5′ AMP. Cardiac AMPK is also under phosphorylation control,
since in vitro incubation of cardiac AMPK with protein phosphatase 2A completely abolished
activity, while incubation with ATP/Mg2+ resulted in over a 2-fold increase in activity. To …
and function of this enzyme in heart muscle has not been characterized. We demonstrate
that rat hearts have a high AMPK activity, comparable to that found in liver, which could be
stimulated up to 3-fold by 5′ AMP. Cardiac AMPK is also under phosphorylation control,
since in vitro incubation of cardiac AMPK with protein phosphatase 2A completely abolished
activity, while incubation with ATP/Mg2+ resulted in over a 2-fold increase in activity. To …