Assay for protein by dye binding
H Van Kley, SM Hale - Analytical Biochemistry, 1977 - Elsevier
H Van Kley, SM Hale
Analytical Biochemistry, 1977•ElsevierA proposed assay for protein [Bradford, MM (1976) Anal. Biochem. 72, 248–254] by binding
of Coomassie brilliant blue G-250 has been evaluated. Some proteins give standard curves
similar to those reported by Bradford, but others deviate widely; caution is urged in
application of the procedure to general assay for protein concentration.
of Coomassie brilliant blue G-250 has been evaluated. Some proteins give standard curves
similar to those reported by Bradford, but others deviate widely; caution is urged in
application of the procedure to general assay for protein concentration.
A proposed assay for protein [Bradford, M. M. (1976) Anal. Biochem. 72, 248–254] by binding of Coomassie brilliant blue G-250 has been evaluated. Some proteins give standard curves similar to those reported by Bradford, but others deviate widely; caution is urged in application of the procedure to general assay for protein concentration.
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