Flow cytometric analysis of cell division history using dilution of carboxyfluorescein diacetate succinimidyl ester, a stably integrated fluorescent probe
AB Lyons, J Hasbold, PD Hodgkin - Methods in cell biology, 2001 - Elsevier
Publisher Summary This chapter reviews the use of a technique based on the serial dilution
of a stably binding intracellular fluorochrome, carboxyfluorescein diacetate succinimidyl
ester (CFSE), which allows eight to ten sequential cell divisions to be analyzed by flow
cytometry. When incubated with cells, the fluorescein-based CFSE crosses the cell
membrane and attaches to free amine groups of cytoplasmic cell proteins. After enzymatic
removal of carboxyl groups by endogenous intracellular esterases, CFSE acquires identical …
of a stably binding intracellular fluorochrome, carboxyfluorescein diacetate succinimidyl
ester (CFSE), which allows eight to ten sequential cell divisions to be analyzed by flow
cytometry. When incubated with cells, the fluorescein-based CFSE crosses the cell
membrane and attaches to free amine groups of cytoplasmic cell proteins. After enzymatic
removal of carboxyl groups by endogenous intracellular esterases, CFSE acquires identical …