Dendritic cells issued in vitro from bone marrow produce PGE2 that contributes to the immunomodulation induced by antigen-presenting cells
H Harizi, M Juzan, C Grosset, M Rashedi, N Gualde - Cellular immunology, 2001 - Elsevier
Given that preliminary work has indicated that prostaglandins can play a role in modulating
dendritic cell (DC) functions, we addressed the prostaglandin E2 (PGE2) biosynthetic
capacity of mouse DC produced in vitro from bone marrow cells. We observed production of
significant amounts of PGE2, which was reduced by at least 80% when cells were incubated
in the presence of indomethacin, a COX-1 preferential inhibitor. Indeed, when tested by
Western blot analysis with specific COX-1 and COX-2 antibodies, only COX-1 expression …
dendritic cell (DC) functions, we addressed the prostaglandin E2 (PGE2) biosynthetic
capacity of mouse DC produced in vitro from bone marrow cells. We observed production of
significant amounts of PGE2, which was reduced by at least 80% when cells were incubated
in the presence of indomethacin, a COX-1 preferential inhibitor. Indeed, when tested by
Western blot analysis with specific COX-1 and COX-2 antibodies, only COX-1 expression …