[HTML][HTML] Phenoxyl free radical formation during the oxidation of the fluorescent dye 2′, 7′-dichlorofluorescein by horseradish peroxidase: possible consequences for …
The oxidation of the fluorescent dye 2′, 7′-dichlorofluorescein (DCF) by horseradish
peroxidase was investigated by optical absorption, electron spin resonance (ESR), and
oxygen consumption measurements. Spectrophotometric measurements showed that DCF
could be oxidized either by horseradish peroxidase-compound I or-compound II with the
obligate generation of the DCF phenoxyl radical (DCF⋅). This one-electron oxidation was
confirmed by ESR spin-trapping experiments. DCF⋅ oxidizes GSH, generating the …
peroxidase was investigated by optical absorption, electron spin resonance (ESR), and
oxygen consumption measurements. Spectrophotometric measurements showed that DCF
could be oxidized either by horseradish peroxidase-compound I or-compound II with the
obligate generation of the DCF phenoxyl radical (DCF⋅). This one-electron oxidation was
confirmed by ESR spin-trapping experiments. DCF⋅ oxidizes GSH, generating the …