[HTML][HTML] Nitric Oxide-stimulated Guanine Nucleotide Exchange on p21ras (∗)
HM Lander, JS Ogiste, SFA Pearce, R Levi… - Journal of Biological …, 1995 - ASBMB
The protooncogene p21 ras, a monomeric G protein family member, plays a critical role in
converting extracellular signals into intracellular biochemical events. Here, we report that
nitric oxide (NO) activates p21 ras in human T cells as evidenced by an increase in GTP-
bound p21 ras. In vitro studies using pure recombinant p21 ras demonstrate that the
activation is direct and reversible. Circular dichroism analysis reveals that NO induces a
profound conformational change in p21 ras in association with GDP/GTP exchange. The …
converting extracellular signals into intracellular biochemical events. Here, we report that
nitric oxide (NO) activates p21 ras in human T cells as evidenced by an increase in GTP-
bound p21 ras. In vitro studies using pure recombinant p21 ras demonstrate that the
activation is direct and reversible. Circular dichroism analysis reveals that NO induces a
profound conformational change in p21 ras in association with GDP/GTP exchange. The …