Oxygenation of polyunsaturated fatty acids during prostaglandin biosynthesis by sheep vesicular glands

WL Smith, WEM Lands - Biochemistry, 1972 - ACS Publications
WL Smith, WEM Lands
Biochemistry, 1972ACS Publications
William L. Smith and William EM Lands* abstract: We found that two types of fatty acid
dioxygenase activity are present in acetone powder preparations of sheep vesicular gland.
One activity (Ea) when stimulated by phenol may be suppressed by the functioning of
glutathione peroxidase. Therefore, Ea seems to require hydroperoxide as an obligatory
intermediate. The oxygenation of fatty acids catalyzed by freshly prepared homogenates of
sheep vesicular gland was also inhibited by glutathione peroxidase in the presence of …
William L. Smith and William E. M. Lands* abstract: We found that two types of fatty acid dioxygenase activity are present in acetone powder preparations of sheep vesicular gland. One activity (Ea) when stimulated by phenol may be suppressed by the functioning of glutathione peroxidase. Therefore, Ea seems to require hydroperoxide as an obligatory intermediate. The oxygenation of fatty acids catalyzed by freshly prepared homogenates of sheep vesicular gland was also inhibited by glutathione peroxidase in the presence of glutathione. The sloweroxygenase activity (Eb) found in untreated vesicular glandacetone powder prepara-tion was not affected by glutathione peroxidase and therefore
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