Histone deacetylases augment cytokine induction of the iNOS gene
Z Yu, W Zhang, BC Kone - Journal of the American Society of …, 2002 - journals.lww.com
The inducible nitric oxide synthase (iNOS) gene plays an important role in renal diseases.
Transcription is the principal mode of regulation. This study explores the role of acetylation
in cytokine-mediated iNOS induction in cultured murine mesangial cells and RAW 264.7
cells. Nitric oxide production was measured by the Griess reaction. The activity of the iNOS
promoter and a nuclear factor–κB (NF-κB) element promoter were assessed in transient
transfection assays. Gel shift and supershift assays were used to identify NF-κB in nuclear …
Transcription is the principal mode of regulation. This study explores the role of acetylation
in cytokine-mediated iNOS induction in cultured murine mesangial cells and RAW 264.7
cells. Nitric oxide production was measured by the Griess reaction. The activity of the iNOS
promoter and a nuclear factor–κB (NF-κB) element promoter were assessed in transient
transfection assays. Gel shift and supershift assays were used to identify NF-κB in nuclear …