[HTML][HTML] Tumor necrosis factor increases hepatocellular glutathione by transcriptional regulation of the heavy subunit chain of γ-glutamylcysteine synthetase
Tumor necrosis factor (TNF) is an inflammatory cytokine that causes cell injury by generation
of oxidative stress. Since glutathione (GSH) is a key cellular antioxidant that detoxifies
reactive oxygen species, the purpose of our work was to examine the regulation of cellular
GSH, the expression of heavy subunit chain of γ-glutamylcysteine synthetase (γ-GCS-HS),
and control of intracellular generation of reactive oxygen species in cultured rat hepatocytes
treated with TNF. Exposure of cells to TNF (10,000 units/ml) resulted in depletion of cellular …
of oxidative stress. Since glutathione (GSH) is a key cellular antioxidant that detoxifies
reactive oxygen species, the purpose of our work was to examine the regulation of cellular
GSH, the expression of heavy subunit chain of γ-glutamylcysteine synthetase (γ-GCS-HS),
and control of intracellular generation of reactive oxygen species in cultured rat hepatocytes
treated with TNF. Exposure of cells to TNF (10,000 units/ml) resulted in depletion of cellular …