[HTML][HTML] Commitment to apoptosis by GD3 ganglioside depends on opening of the mitochondrial permeability transition pore
We have studied the effects of GD3 ganglioside on mitochondrial function in isolated
mitochondria and intact cells. In isolated mitochondria, GD3 ganglioside induces complex
changes of respiration that depend on the substrate being oxidized. However, these effects
are secondary to opening of the cyclosporin A-sensitive permeability transition pore and to
the ensuing swelling and cytochromec depletion rather than to an interaction with the
respiratory chain complexes. By using a novel in situ assay based on the fluorescence …
mitochondria and intact cells. In isolated mitochondria, GD3 ganglioside induces complex
changes of respiration that depend on the substrate being oxidized. However, these effects
are secondary to opening of the cyclosporin A-sensitive permeability transition pore and to
the ensuing swelling and cytochromec depletion rather than to an interaction with the
respiratory chain complexes. By using a novel in situ assay based on the fluorescence …