[HTML][HTML] Characterization of the AMP-activated protein kinase kinase from rat liver and identification of threonine 172 as the major site at which it phosphorylates AMP …
SA Hawley, M Davison, A Woods, SP Davies… - Journal of Biological …, 1996 - ASBMB
We have developed a sensitive assay for the AMP-activated protein kinase kinase, the
upstream component in the AMP-activated protein kinase cascade. Phosphorylation and
activation of the downstream kinase by the upstream kinase absolutely requires AMP and is
antagonized by high (millimolar) concentrations of ATP. We have purified the upstream
kinase> 1000-fold from rat liver; a variety of evidence indicates that the catalytic subunit may
be a polypeptide of 58 kDa. The physical properties of the downstream and upstream …
upstream component in the AMP-activated protein kinase cascade. Phosphorylation and
activation of the downstream kinase by the upstream kinase absolutely requires AMP and is
antagonized by high (millimolar) concentrations of ATP. We have purified the upstream
kinase> 1000-fold from rat liver; a variety of evidence indicates that the catalytic subunit may
be a polypeptide of 58 kDa. The physical properties of the downstream and upstream …