[HTML][HTML] Mitochondria recycle Ca2+ to the endoplasmic reticulum and prevent the depletion of neighboring endoplasmic reticulum regions* 210
S Arnaudeau, WL Kelley, JV Walsh… - Journal of biological …, 2001 - ASBMB
To study Ca 2+ fluxes between mitochondria and the endoplasmic reticulum (ER), we used"
cameleon" indicators targeted to the cytosol, the ER lumen, and the mitochondrial matrix.
High affinity mitochondrial probes saturated in∼ 20% of mitochondria during histamine
stimulation of HeLa cells, whereas a low affinity probe reported averaged peak values of
106±5 µm, indicating that Ca 2+ transients reach high levels in a fraction of mitochondria. In
concurrent ER measurements,[Ca 2+] ER averaged 371±21 µm at rest and decreased to …
cameleon" indicators targeted to the cytosol, the ER lumen, and the mitochondrial matrix.
High affinity mitochondrial probes saturated in∼ 20% of mitochondria during histamine
stimulation of HeLa cells, whereas a low affinity probe reported averaged peak values of
106±5 µm, indicating that Ca 2+ transients reach high levels in a fraction of mitochondria. In
concurrent ER measurements,[Ca 2+] ER averaged 371±21 µm at rest and decreased to …