[PDF][PDF] Interferon gamma plays a critical role in T cell-dependent liver injury in mice initiated by concanavalin A

S Kusters, F Gantner, G Kunstle… - GASTROENTEROLOGY …, 1996 - academia.edu
S Kusters, F Gantner, G Kunstle, G Tiegs
GASTROENTEROLOGY-BALTIMORE THEN PHILADELPHIA-, 1996academia.edu
Background & Aims: T cell–dependent liver injury in- receptor deficiency5 prevented mice
from LPS lethality. volving endogenous tumor necrosis factor (TNF) a can These effects were
mediated by enhanced2 or reduced5, 6 be induced by either concanavalin A in naive mice
or TNF production, respectively. Natural killer cells were by activating anti-CD3 antibody or
staphylococcal en- suggested as the cellular source of LPS-induced IFN-g, terotoxin B in D-
galactosamine–sensitized mice. In this which in turn primes macrophages to produce …
Background & Aims: T cell–dependent liver injury in- receptor deficiency5 prevented mice from LPS lethality. volving endogenous tumor necrosis factor (TNF) a can These effects were mediated by enhanced2 or reduced5, 6 be induced by either concanavalin A in naive mice or TNF production, respectively. Natural killer cells were by activating anti-CD3 antibody or staphylococcal en- suggested as the cellular source of LPS-induced IFN-g, terotoxin B in D-galactosamine–sensitized mice. In this which in turn primes macrophages to produce enhanced study, the role of interferon gamma (IFN-g) in these T-amounts of TNF. 1 In a T cell–dependent model, ie, cell models was addressed. Methods: Mice were pre-staphylococcal enterotoxin B (SEB)-induced shock in N-treated with a neutralizing anti-mouse IFN-g antiserum nitro-L-arginine methyl ester–treated mice, TNF as well before injection of T cell–activating agents. Plasma as IFN-g were shown to mediate lethality. 7 cytokine and transaminase levels were determined. In liver injury, increased production of IFN-g was
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