[7] Chemical procedures for analysis of polysaccharides

WZ Hassid, S Abraham - 1957 - Elsevier
Publisher Summary This chapter presents a procedure for the determination of glycogen
and starch. Determination of glycogen by the modified Pflüger method consist digestion of
the tissue in hot concentrated KOH, precipitation of the glycogen with ethanol, hydrolysis of
the glycogen with acid, and determination of the glucose in the hydrolyzate as reducing
sugar. Determination of glycogen with anthrone reagent includes digestion with 30% KOH.
The glycogen is treated with the anthrone reagent and determined colorimetrically as …