Allergens of wild house dust mites: environmental Der p 1 and Der p 2 sequence polymorphisms
WA Smith, BJ Hales, AG Jarnicki… - Journal of allergy and …, 2001 - Elsevier
Background: Sequence diversity is a common feature of mite allergens. Previous studies,
using predominantly commercial mite clones, have described several polymorphic residues
for Der p 1 and Der p 2. Objective: This study aimed at determining the occurrence of
sequence diversity in environmental mite isolates. Methods: Mites were isolated from
houses in Perth and Sydney, Australia. Total RNA was extracted from 1 to 30 Perth mites,
and cDNA was synthesized by reverse transcriptase PCR. Der p 1 and Der p 2 cDNAs were …
using predominantly commercial mite clones, have described several polymorphic residues
for Der p 1 and Der p 2. Objective: This study aimed at determining the occurrence of
sequence diversity in environmental mite isolates. Methods: Mites were isolated from
houses in Perth and Sydney, Australia. Total RNA was extracted from 1 to 30 Perth mites,
and cDNA was synthesized by reverse transcriptase PCR. Der p 1 and Der p 2 cDNAs were …