[HTML][HTML] The Escherichia coli OxyS regulatory RNA represses fhlA translation by blocking ribosome binding
S Altuvia, A Zhang, L Argaman, A Tiwari… - The EMBO journal, 1998 - embopress.org
OxyS is a small untranslated RNA which is induced in response to oxidative stress in
Escherichia coli. This novel RNA acts as a global regulator to activate or repress the
expression of as many as 40 genes, including the fhlA‐encoded transcriptional activator and
the rpoS‐encoded σ s subunit of RNA polymerase. Deletion analysis of OxyS showed that
different domains of the small RNA are required for the regulation of fhlA and rpoS. We
examined the mechanism of OxyS repression of fhlA and found that the OxyS RNA inhibits …
Escherichia coli. This novel RNA acts as a global regulator to activate or repress the
expression of as many as 40 genes, including the fhlA‐encoded transcriptional activator and
the rpoS‐encoded σ s subunit of RNA polymerase. Deletion analysis of OxyS showed that
different domains of the small RNA are required for the regulation of fhlA and rpoS. We
examined the mechanism of OxyS repression of fhlA and found that the OxyS RNA inhibits …
Abstract
OxyS is a small untranslated RNA which is induced in response to oxidative stress in Escherichia coli. This novel RNA acts as a global regulator to activate or repress the expression of as many as 40 genes, including the fhlA‐encoded transcriptional activator and the rpoS‐encoded σ s subunit of RNA polymerase. Deletion analysis of OxyS showed that different domains of the small RNA are required for the regulation of fhlA and rpoS. We examined the mechanism of OxyS repression of fhlA and found that the OxyS RNA inhibits fhlA translation by pairing with a short sequence overlapping the Shine–Dalgarno sequence, thereby blocking ribosome binding/translation.
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