[HTML][HTML] Intracellular trafficking of cholesterol monitored with a cyclodextrin
EB Neufeld, AM Cooney, J Pitha… - Journal of Biological …, 1996 - ASBMB
The sterol binding agent 2-hydroxypropyl-β-cyclodextrin is shown to be a convenient and
useful experimental tool to probe intracellular pathways of cholesterol transport. Biochemical
and cytochemical studies reveal that cyclodextrin specifically removes plasma membrane
cholesterol. Depletion of plasma membrane sphingomyelin greatly accelerated cyclodextrin-
mediated cholesterol removal. Cholesterol arriving at the plasma membrane from lysosomes
and the endoplasmic reticulum was also removed by cyclodextrin. Cellular cholesterol …
useful experimental tool to probe intracellular pathways of cholesterol transport. Biochemical
and cytochemical studies reveal that cyclodextrin specifically removes plasma membrane
cholesterol. Depletion of plasma membrane sphingomyelin greatly accelerated cyclodextrin-
mediated cholesterol removal. Cholesterol arriving at the plasma membrane from lysosomes
and the endoplasmic reticulum was also removed by cyclodextrin. Cellular cholesterol …