[HTML][HTML] SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box
Cell, 1996•cell.com
We have identified the yeast and human homologs of the SKP1 gene as a suppressor of
cdc4 mutants and as a cyclin F–binding protein. Skp1p indirectly binds cyclin A/Cdk2
through Skp2p, and directly binds Skp2p, cyclin F, and Cdc4p through a novel structural
motif called the F-box. SKP1 is required for ubiquitin-mediated proteolysis of Cln2p, Clb5p,
and the Cdk inhibitor Sic1p, and provides a link between these molecules and the
proteolysis machinery. A large number of proteins contain the F-box motif and are thereby …
cdc4 mutants and as a cyclin F–binding protein. Skp1p indirectly binds cyclin A/Cdk2
through Skp2p, and directly binds Skp2p, cyclin F, and Cdc4p through a novel structural
motif called the F-box. SKP1 is required for ubiquitin-mediated proteolysis of Cln2p, Clb5p,
and the Cdk inhibitor Sic1p, and provides a link between these molecules and the
proteolysis machinery. A large number of proteins contain the F-box motif and are thereby …
Abstract
We have identified the yeast and human homologs of the SKP1 gene as a suppressor of cdc4 mutants and as a cyclin F–binding protein. Skp1p indirectly binds cyclin A/Cdk2 through Skp2p, and directly binds Skp2p, cyclin F, and Cdc4p through a novel structural motif called the F-box. SKP1 is required for ubiquitin-mediated proteolysis of Cln2p, Clb5p, and the Cdk inhibitor Sic1p, and provides a link between these molecules and the proteolysis machinery. A large number of proteins contain the F-box motif and are thereby implicated in the ubiquitin pathway. Different skp1 mutants arrest cells in either G1 or G2, suggesting a connection between regulation of proteolysis in different stages of the cycle.
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