[HTML][HTML] Insolubility of lipids in triton X-100: physical origin and relationship to sphingolipid/cholesterol membrane domains (rafts)
E London, DA Brown - Biochimica et Biophysica Acta (BBA)-Biomembranes, 2000 - Elsevier
The insolubility of lipids in detergents is a useful method for probing the structure of
biological membranes. Insolubility in detergents like Triton X-100 is observed in lipid
bilayers that exist in physical states in which lipid packing is tight. The Triton X-100-insoluble
lipid fraction obtained after detergent extraction of eukaryotic cells is composed of detergent-
insoluble membranes rich in sphingolipids and cholesterol. These insoluble membranes
appear to arise from sphingolipid-and cholesterol-rich membrane domains (rafts) in the …
biological membranes. Insolubility in detergents like Triton X-100 is observed in lipid
bilayers that exist in physical states in which lipid packing is tight. The Triton X-100-insoluble
lipid fraction obtained after detergent extraction of eukaryotic cells is composed of detergent-
insoluble membranes rich in sphingolipids and cholesterol. These insoluble membranes
appear to arise from sphingolipid-and cholesterol-rich membrane domains (rafts) in the …