[HTML][HTML] Phosphatase inhibition leads to histone deacetylases 1 and 2 phosphorylation and disruption of corepressor interactions

SC Galasinski, KA Resing, JA Goodrich… - Journal of biological …, 2002 - ASBMB
The regulation of histone deacetylases (HDACs) by phosphorylation was examined by
elevating intracellular phosphorylation in cultured cells with the protein phosphatase
inhibitor okadaic acid. After fractionation of extracts from treated versus untreated cells,
HDAC 1 and 2 eluted in several peaks of deacetylase activity, assayed using mixed
acetylated histones or acetylated histone H4 peptide. Stimulation of cells with okadaic acid
led to hyperphosphorylation of HDAC 1 and 2 as well as changes in column elution of both …