Characterization of U46619 binding in unactivated, intact human platelets and determination of binding site affinities of four TXA2/PGH2 receptor antagonists (13-APA …
EJ Kattelman, DL Venton, GC Le Breton - Thrombosis research, 1986 - Elsevier
The binding of U46619 and the inhibition of this binding by four TXA 2/PGH 2 receptor
antagonists (13-APA, BM 13.177, ONO 3708 and SQ 29, 48) were studied in unactivate,
intact human platelets. Washed platelets were equilibrated with [3 h]-U46619 (5 nM) and the
time course of binding determined. The receptor-specific binding reached equilibrium within
2–4 minutes, and could be displaced by addition of excess unlabelled ligand. Saturation of
this binding was achieved at 750 nM. Scatchard transformation of the saturation binding …
antagonists (13-APA, BM 13.177, ONO 3708 and SQ 29, 48) were studied in unactivate,
intact human platelets. Washed platelets were equilibrated with [3 h]-U46619 (5 nM) and the
time course of binding determined. The receptor-specific binding reached equilibrium within
2–4 minutes, and could be displaced by addition of excess unlabelled ligand. Saturation of
this binding was achieved at 750 nM. Scatchard transformation of the saturation binding …