Nucleotide sequence of the herpes simplex virus type 2 (HSV-2) thymidine kinase gene and predicted amino acid sequence of thymidine kinase polypeptide and its …
S Kit, M Kit, H Qavi, D Trkula, H Otsuka - Biochimica et Biophysica Acta …, 1983 - Elsevier
To analyze the boundaries of the functional coding region of the HSV-2 (333) thymidine
kinase gene (TK gene), deletion mutants of hybrid plasmid pMAR401 H2G, which contains
the 17.5 kbp BglII-G fragment of HSV-2 DNA, were prepared and tested for capacity to
transform LM (TK−) cells to the thymidine kinase-positive phenotype. These studies showed
that hybrid plasmids containing 2.2–2.4 kbp subfragments of HSV-2 BglII-G DNA
transformed LM (TK−) cells to the thymidine kinase-positive phenotype and suggested that …
kinase gene (TK gene), deletion mutants of hybrid plasmid pMAR401 H2G, which contains
the 17.5 kbp BglII-G fragment of HSV-2 DNA, were prepared and tested for capacity to
transform LM (TK−) cells to the thymidine kinase-positive phenotype. These studies showed
that hybrid plasmids containing 2.2–2.4 kbp subfragments of HSV-2 BglII-G DNA
transformed LM (TK−) cells to the thymidine kinase-positive phenotype and suggested that …