Identification and cloning of a megakaryocyte growth and development factor that is a ligand for the cytokine receptor MpI
TD Bartley, J Bogenberger, P Hunt, YS Li, HS Lu… - Cell, 1994 - cell.com
TD Bartley, J Bogenberger, P Hunt, YS Li, HS Lu, F Martin, MS Chang, B Samal, JL Nichol…
Cell, 1994•cell.comA novel megakaryocyte growth and development factor (MGDF) has been identified in
aplastic canine plasma, and its cDNAs have been cloned from canine, murine, and human
sources. Purlfied canine MGDF isolated by procedures Involving Mpl receptor afflnlty
chromatography exists In at least two forms, with ap parent molecular masses of 25 kDa and
31 kDa, that share the N-terminal amino acid sequence APPACDPRLLNKMLRDSHVLH.
Human, dog, and mouse cDNAs for MGDF are highly conserved and encode open reading …
aplastic canine plasma, and its cDNAs have been cloned from canine, murine, and human
sources. Purlfied canine MGDF isolated by procedures Involving Mpl receptor afflnlty
chromatography exists In at least two forms, with ap parent molecular masses of 25 kDa and
31 kDa, that share the N-terminal amino acid sequence APPACDPRLLNKMLRDSHVLH.
Human, dog, and mouse cDNAs for MGDF are highly conserved and encode open reading …
Summary
A novel megakaryocyte growth and development factor (MGDF) has been identified in aplastic canine plasma, and its cDNAs have been cloned from canine, murine, and human sources. Purlfied canine MGDF isolated by procedures Involving Mpl receptor afflnlty chromatography exists In at least two forms, with ap parent molecular masses of 25 kDa and 31 kDa, that share the N-terminal amino acid sequence APPACDPRLLNKMLRDSHVLH. Human, dog, and mouse cDNAs for MGDF are highly conserved and encode open reading frames for protelns of 353,352, and 356 amino acids, respectively, including predicted slgnal peptkfes. Canine MGDF and recombinant human MGDF support the development of megakaryocytes from human CD34+ progenltor cell populations in liquid culture and promote the survival of a factor-dependent murine cell line (32D) engineered to express Mpl. These biological activities are blocked by the soluble extracellular domain of Mpl. These data demonstrate that MGDF is a novel cytoklne that regulates megakaryocyte development and isa ligand forthe MPI receptor.
cell.com