[HTML][HTML] Oligomerization of the human thyrotropin receptor: fluorescent protein-tagged hTSHR reveals post-translational complexes

R Latif, P Graves, TF Davies - Journal of Biological Chemistry, 2001 - ASBMB
To examine thyrotropin (TSH) receptor homophilic interactions we fused the human TSH
receptor (hTSHR) carboxyl terminus to green fluorescent protein (GFP) and the
corresponding chimeric cDNA was expressed in Chinese hamster ovary cells. Fluorescent
TSH receptors on the plasma membrane were functional as assessed by TSH-induced
cAMP synthesis. The binding of TSH, as well as TSHR autoantibodies, induced time-and
dose-dependent receptor capping. Fluorescence resonance energy transfer between …