Expression of herpes simplex virus 1 glycoprotein B by a recombinant vaccinia virus and protection of mice against lethal herpes simplex virus 1 infection.
EM Cantin, R Eberle, JL Baldick… - Proceedings of the …, 1987 - National Acad Sciences
EM Cantin, R Eberle, JL Baldick, B Moss, DE Willey, AL Notkins, H Openshaw
Proceedings of the National Academy of Sciences, 1987•National Acad SciencesThe herpes simplex virus 1 (HSV-1) strain F gene encoding glycoprotein gB was isolated
and modified at the 5'end by in vitro oligonucleotide-directed mutagenesis. The modified gB
gene was inserted into the vaccinia virus genome and expressed under the control of a
vaccinia virus promoter. The mature gB glycoprotein produced by the vaccinia virus
recombinant was glycosylated, was expressed at the cell surface, and was indistinguishable
from authentic HSV-1 gB in terms of electrophoretic mobility. Mice immunized intradermally …
and modified at the 5'end by in vitro oligonucleotide-directed mutagenesis. The modified gB
gene was inserted into the vaccinia virus genome and expressed under the control of a
vaccinia virus promoter. The mature gB glycoprotein produced by the vaccinia virus
recombinant was glycosylated, was expressed at the cell surface, and was indistinguishable
from authentic HSV-1 gB in terms of electrophoretic mobility. Mice immunized intradermally …
The herpes simplex virus 1 (HSV-1) strain F gene encoding glycoprotein gB was isolated and modified at the 5' end by in vitro oligonucleotide-directed mutagenesis. The modified gB gene was inserted into the vaccinia virus genome and expressed under the control of a vaccinia virus promoter. The mature gB glycoprotein produced by the vaccinia virus recombinant was glycosylated, was expressed at the cell surface, and was indistinguishable from authentic HSV-1 gB in terms of electrophoretic mobility. Mice immunized intradermally with the recombinant vaccinia virus produced gB-specific neutralizing antibodies and were resistant to a lethal HSV-1 challenge.
National Acad Sciences