[HTML][HTML] Expression cloning of an oxysterol 7α-hydroxylase selective for 24-hydroxycholesterol
J Li-Hawkins, EG Lund, AD Bronson… - Journal of Biological …, 2000 - ASBMB
The synthesis of 7α-hydroxylated bile acids from oxysterols requires an oxysterol 7α-
hydroxylase encoded by theCyp7b1 locus. As expected, mice deficient in this enzyme have
elevated plasma and tissue levels of 25-and 27-hydroxycholesterol; however, levels of
another major oxysterol, 24-hydroxycholesterol, are not increased in these mice, suggesting
the presence of another oxysterol 7α-hydroxylase. Here, we describe the cloning and
characterization of murine and human cDNAs and genes that encode a second oxysterol 7α …
hydroxylase encoded by theCyp7b1 locus. As expected, mice deficient in this enzyme have
elevated plasma and tissue levels of 25-and 27-hydroxycholesterol; however, levels of
another major oxysterol, 24-hydroxycholesterol, are not increased in these mice, suggesting
the presence of another oxysterol 7α-hydroxylase. Here, we describe the cloning and
characterization of murine and human cDNAs and genes that encode a second oxysterol 7α …