We have used restriction isotyping (restriction enzyme isoform genotyping) for rapid typing of
common apolipoprotein E isoforms (E2, E3, E4). ApoE restriction isotyping used
oligonucleotides to amplify apolipoprotein E gene sequences containing amino acid
positions 112 and 158. The amplification products were digested with HhaI and subjected to
electrophoresis on polyacrylamide gels. Each of the isoforms was distinguished by a unique
combination of HhaI fragment sizes that enabled unambiguous typing of all homozygotic …