Plasminogen activation by receptor-bound urokinase. A kinetic study with both cell-associated and isolated receptor

V Ellis, N Behrendt, K Danø - Journal of Biological Chemistry, 1991 - ASBMB
The specific cellular receptor for urokinase-type plasminogen activator (uPA) is found on a
variety of cell types and has been postulated to play a central role in the mediation of
pericellular proteolytic activity. We have studied the kinetics of plasminogen (Plg) activation
catalyzed by uPA specifically bound to its receptor on the human monocytoid cell-line U937
and demonstrate this process to have properties differing widely from those observed for
uPA in solution. The solution-phase reaction was characterized by a Km of 25 microM and …