Reconstitution of functional water channels in liposomes containing purified red cell CHIP28 protein

ML Zeidel, SV Ambudkar, BL Smith, P Agre - Biochemistry, 1992 - ACS Publications
Biochemistry, 1992ACS Publications
Reconstitution of functional water channels in liposomes containing purified red cell
CHIP28 protein Page 1 7436 Biochemistry 1992, 31, 7436-7440 Accelerated Publications
Reconstitution of Functional Water Channels in Liposomes Containing Purified Red Cell
CHIP28 Protein* Mark L. Zeidel,* Suresh V. Ambudkar,8-# Barbara L. Smith,8-* 1 IIand
Peter Agre*-8-1 Medical Service, West Roxbury Department of Veterans Affairs Medical
Center, and Department of Medicine, Brigham and Women's and Children’s Hospitals …
MATERIALS AND METHODS
Materials. Octyl glucoside (octyl 0-D-glucopyranoside) was obtained from Calbiochem. Triton X-100, dithiothreitol, PMSF (phenylmethanesulfonyl fluoride), and TV-lauroylsarcosine were from Sigma. Carboxyfluorescein was from Mo-
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